From Padua's main station, take local public transport toward Palazzo del Bo. Get off at the nearest stop and walk the final few minutes to Palazzo del Bo. Check the current local connection on the day of travel.
From Orto Botanico, walk to the nearest public transport stop and travel back to Padua's main station. The departure point is Orto Botanico in Via Orto Botanico. Check the current local connection on the day of travel.